Can you inoculate with mycelium

Please advise. As a first time grower starting from liquid culture i learned quickly about having a sterile environment. I broke down and built a decent size laminar hood, build a dedicated lab and following the same process, as did prior. I was able to generate uncontaminated grain spawn. Can anything bad happen if you use way too much liquid spore solution?

A friend of mine put a whole syringe in a bag that only needs ten ccs. Im just starting i have 9 dif strains of sporrs and my 1st and secnd try with bags they turned a yellow.

Heat around 72 in cupboard near ground and i did not have inoc ports in em so went threw the heppa filter is it that lil hole i make in it that the contaminates getting in. You should definitely close the little hole where you inoculated with some tape. My guess is that the bags were not sterilized properly or the spore syringes were not clean. Maybe increase the time of your sterilization. I used a 5 pound mushroom bag with grain and everything inside.

It took 4 weeks but the entire thing turned white with yellow water areas. With that said, some of the non white color was from the dirt in the bag. It carries nice stuff. I loved as much as you will receive carried out right here.

The sketch is attractive, your authored subject matter stylish. So I have my mono tub all set up and ran a test run with no substrate or anything in tub. I have a separate tub with fogged and 4 inch duct fan blowing fog into bigger tub. My humidity was perfect for 3 days continuously but no condensation on walls of tub would the substrate dry out or will the humidified air be sufficient. Hi there, I need an advice for indsutrial oyster mushrooming. Our land is very close to a dam and its forbidden covering concrete for the tents.

Is there any alternative and sanity flooring other than concrete? Hey so this is my second time trying to grow the first I tried to do it with APE culture I got online which I think could of been contaminated.

So I threw them out and tried agian with a cream lex Luther culture that I made in between the first and second go around. My 60cc spore syringe got clogged while inoculating one of my grain bags on the second time around and I accidently squeezed 10ccs of it in a 3lb bad.

Am I doing something wrong or do I just need to leave it and be more patient or rotate it or what? Hi, Its been 9 days since I inoculated my jars. They jars are kept in a large plastic box, with the lit slightly off.

The room has about 27 degrees celcius. I can only see a little white mycelium in one of the jars. What am I doing wrong? Thank you. I love Mushroom a great deal.

It shows up as often as possible on my dishes however in market, mushrooms are costly. So I am truly giving genuine idea to develop it in my home.

Always wait until full cultivation or you increase the chance of contamination during fruiting. Patience is key. I have several jars that turned partly black as the white mycelium were developing. Within days the black disappeared and the white mycelium took completely over with no sign of any black in the jars.

Are the white mycelium contaminated or can I take these jars to the next growing level and will the mushrooms be safe to use? You might be lucky and have good finish but next time cut of the black spot with sterile instrument. I have had a few jars of inoculated rye berries that never form mycelium.

The grains seem wet, which suggests that I injected too much spore solution. Is there a way to reduce the humidity in the jars at this point? Or should I just start over? Unless your growing pan cyans i dont know why your humidity is so high…the puddles are fine. Put the fogger on a wifi timer…. Hi, I see standing water at the bottom of my oyster mushroom bags and also a lot Of water droplets.

I tried to cut the bottom a little and tried to drain the water with a help of some cotton. Will that help? Thank you! I know…. Grain on the bottom straw in the middle and horse manure substrate on top. My problem is most of my shrooms are growing in the straw and not on top. What do I do?? Should I cut the bag or just let them grow like that?? The kit came with the mycellum in the bag n compost separate to place on top..

I think my mistake was I didnt materialise the knife.. N if I remove the mold with sterile equipment should I have anouther go at just topping up the compost or does anyone suggest to remove the mycellum n place it in a sterile container n then topped with compost? Or hay? I continued to next process and 2 more weeks later, still nothing.

Looks like its growing a green mold on the substrate. Should I toss this kit and give up? My golden teachers are growing well but on some of the mushrooms there are fuzzy white-ish tips at the tops. What would cause this? Thank you!!! If I got brown sludge on my mycelium in my container how do I prevent that next time.

Was it cause my container was on the floor? Or was it cause the temp in my closet was to high, which I noticed afterwards. My spores have already turned my pasteurized subtrate completely white after 5 days. Should I wait to cast for another week? I am seeing a creamy, milky substance at the bottom of my grow bag, after a week after inoculation. What might this be? Your email address will not be published. Learning how to grow mushrooms at home can be intimidating at first.

In the world of mushroom cultivation, contamination is as inevitable as death and taxes. All rights Reserved. Username or Email Address. Remember Me. Password: Confirm Password:. Sign up for our mailing list and be the first to know about promotions, events, and new product releases. Fall is creeping up on us here in Maine.

Days are getting shorter, shadows longer, and the night air is crisp. Those of you who experience milder winters can grow mushrooms all year. Follow along for some late season ideas and tips for different regions.

Mushroom beds can be installed anytime during the year, as long as the mycelium has a couple of weeks to become established before freezing temperatures hit. This includes wine caps and most of our oyster mushrooms. As heat-loving mushrooms, pink oysters are perennial only in subtropical and tropical regions but grow well as annuals during the summer months of colder zones.

With any luck, spring rains will bring luscious mushroom flushes! Curious how to make mushroom beds? Click here. Wine cap and oysters will both do well. Similar to mushroom beds, containers can be inoculated almost anytime. Using this method is a great way to grow mushrooms at home with limited space and without specialized equipment. Growing cold-hardy mushrooms like blue oysters , snow oysters , or italian oysters is easy in the fall! Depending on how much spawn you use, how big your container is, and the temperature, it should be fully colonized in about a month.

The cold snaps of fall often trigger fruitings, mimicking natural cycles. If temperatures dip below freezing consistently, the mycelium will go dormant. You can bring your container inside to keep growing through winter or give it some protection from the elements outside and wait until the spring for more flushes.

The fall season offers some advantages for cutting logs, especially in warmer regions. Except for hemlock reishi , all tree species used for log inoculation are deciduous. Unlike conifers, deciduous trees go through a period of dormancy coinciding with shorter days and cooler temperatures. We may notice this when leaves change color and drop.

Inside the tree, nutrients are being transferred from the foliage down into stems and trunks for storage. To prevent cracking from freezing temperatures, cells harden and replace some water content with sugars. In the spring, those nutrients and sugary sap flow back up in the tree to supply leaf and flower buds with enough energy to burst open and unfold.

While logs can be cut and inoculated nearly any time of year, strategic timing can increase yield and longevity. Liquid culture consists of a distilled mixture of water and nutrients. Commonly used nutrient sources include Karo and honey.

Nutrients and water allow spores to germinate and begin mycellial growth before inoculation. Many online vendors will only sell edibles inoculate in culture syringes or slants. Agar wedge When cloning mushroom tissue or isolating a strain , the work is usually done on agar plates. From these plates, the tissue can either be dropped into a liquid culture , or more commonly, directly into a spawn substrate jar.

Grain-to-grain transfer G2G In order to quickly increase the amount colonized spawn substrate, a colonized grain jar can be shaken and transferred to other, un-colonized jars. Since a small amount of kernels can effectively inoculate a whole new jar of grain, grain-to-grain transferring can dramatically increase the amount of available spawn substrate. Online retailers may carry grain spawn or "plugs," inoculated wooden dowels used to colonize solid wood substrates.

Bulk substrates are rarely directly inoculated with spores or tissue culture. They are almost always inoculated with a spawn substrate in a process commonly referred to as "spawning".